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- Yes, date unknown
 | Philip II Habsburg, King of Spain 1527-1598 |
| Philip II Habsburg, King of Spain | |
| B: | 21 May 1527 |
| Valladolid, Valladolid, Spain | |
| D: | 13 Sep 1598 |
| El Escorial, El Escorial, Madrid, Spain | |
| Unknown |
| Elizabeth de Valois, Princess -Yes, date unknown |
| Elizabeth de Valois, Princess | |
| B: | France |
| M: | 1559 |
| D: | Yes, date unknown |
| Maria -Yes, date unknown |
| Maria | |
| B: | Portugal |
| M: | 1543 |
| D: | Yes, date unknown |
| Mary I "Bloody Mary" Tudor, Queen of England | |
| B: | 18 Feb 1516 |
| London, Middlesex, England | |
| M: | 25 Jul 1554 |
| England | |
| D: | 17 Nov 1558 |
| London, Middlesex, England | |
| Charles V Habsburg, Emperor 1500-1558 |
| Charles V Habsburg, Emperor | |
| B: | 24 Feb 1500 |
| Gent, East Flanders, Belgium | |
| D: | 21 Sep 1558 |
| San Jeronimo, de Yuste, Extremadura, Spain | |
| Isabella -Yes, date unknown |
| Isabella | |
| B: | Portugal |
| D: | Yes, date unknown |
| Habsburg -Yes, date unknown |
| Habsburg | |
| D: | Yes, date unknown |
| Ferdinand I Habsburg, Emperor 1503-1564 |
| Ferdinand I Habsburg, Emperor | |
| B: | 10 Mar 1503 |
| Alcala de Henares, Madrid, Spain | |
| D: | 25 Jul 1564 |
| Vienna, Lower Austria, Austria | |
| Philip I Habsburg, King of Castile 1478-1506 |
| Philip I Habsburg, King of Castile | |
| B: | 1478 |
| Bruges, West Flanders, Belgium | |
| D: | 1506 |
| Spain | |
| Joanna "The Mad", Queen of Castile -Yes, date unknown |
| Joanna "The Mad", Queen of Castile | |
| B: | Spain |
| M: | 1496 |
| D: | Yes, date unknown |
|
| Mary, Duchess -Yes, date unknown |
| Mary, Duchess | |
| B: | France |
| D: | Yes, date unknown |
| Maximilian I Habsburg, Emperor 1459-1519 |
| Maximilian I Habsburg, Emperor | |
| B: | 1459 |
| Weiner Neustadt, Lower Austria, Austria | |
| M: | 1477 |
| D: | 1519 |
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