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- Yes, date unknown
| Charles V Habsburg, Emperor | |
| B: | 24 Feb 1500 |
| Gent, East Flanders, Belgium | |
| D: | 21 Sep 1558 |
| San Jeronimo, de Yuste, Extremadura, Spain | |
| Isabella | |
| B: | Portugal |
| D: | Yes, date unknown |
| Ferdinand I Habsburg, Emperor | |
| B: | 10 Mar 1503 |
| Alcala de Henares, Madrid, Spain | |
| D: | 25 Jul 1564 |
| Vienna, Lower Austria, Austria | |
| Joanna "The Mad", Queen of Castile | |
| B: | Spain |
| D: | Yes, date unknown |
| Philip I Habsburg, King of Castile | |
| B: | 1478 |
| Bruges, West Flanders, Belgium | |
| M: | 1496 |
| D: | 1506 |
| Spain | |
| Katherine "of Aragon", Queen Consort | |
| B: | 1485 |
| Aragon, Spain | |
| D: | 6 Jan 1536 |
| Kimbalton, England | |
| Arthur Tudor, Prince of Wales | |
| B: | Winchester, Hampshire, England |
| M: | Nov 1501 |
| England | |
| D: | 12 Mar 1502 |
| England | |
| Henry Tudor, Prince | |
| D: | Yes, date unknown |
| Mary I "Bloody Mary" Tudor, Queen of England | |
| B: | 18 Feb 1516 |
| London, Middlesex, England | |
| D: | 17 Nov 1558 |
| London, Middlesex, England | |
| Philip II Habsburg, King of Spain | |
| B: | 21 May 1527 |
| Valladolid, Valladolid, Spain | |
| M: | 25 Jul 1554 |
| England | |
| D: | 13 Sep 1598 |
| El Escorial, El Escorial, Madrid, Spain | |
| Henry VIII Tudor, King of England | |
| B: | 28 Jun 1491 |
| London, Middlesex, England | |
| M: | 3 Jun 1509 |
| England | |
| D: | 28 Jan 1547 |
| London, Middlesex, England | |
| Isabella I "The Catholic", Queen of Castile | |
| B: | 1451 |
| Spain | |
| D: | 1504 |
| Spain | |
| Ferdinand II "The Catholic", King of Castile | |
| B: | 1452 |
| Spain | |
| M: | 1469 |
| Spain | |
| D: | 1516 |
| Isabella, Princess | |
| B: | Portugal |
| D: | Yes, date unknown |
| Juan II, King of Castile | |
| B: | 1405 |
| Spain | |
| M: | 1450 |
| D: | 1454 |
| Spain | |
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